Composite
Part:BBa_K1157020
Designed by: Lin Ang Chieh Group: iGEM13_NTU-Taida (2013-09-15)
Pc-RhlR-pRhl-RhlR-GFPmut3
This biosensor is built to sense C-4 AHL molecules. The constitutive promoter continues to express Rhl receptor(RhlR), and as the Rhl receptor binds with C-4 AHL, it binds with promoter sequence of Rhl and produces more Rhl receptor and reporter GFP which can be detected. The adding of a Rhl receptor before GFP is to induce a positive-feedback loop, for magnifying signal.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 304
Illegal BamHI site found at 1260 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 779
Illegal BsaI site found at 1735
Illegal BsaI.rc site found at 2422
[edit]
Categories
Parameters
None |